With diverse, global collections and research in art, culture, and natural history, the Royal Ontario Museum is ideally positioned to help address humanity's urgent, multidimensional environmental challenges, such as climate change and biodiversity loss. ROM contributes, in part, by facilitating transdisciplinary collaborations between artists and scientists. The 'Art of the Expedition' illustrates such a collaboration, in which ROM's Curator of Fishes Dr. Nathan Lujan work's closely with New York-based artist David Brooks to bring their respective expertise to bear on the issues of species discovery, classification, and loss in Peru's western headwaters of the Amazon Basin, Earth's most biodiverse freshwater ecosystem. Throughout Nathan's career, he has discovered and described as new to science over three dozen fish species and has worked to understand the ecological and evolutionary relationships among Amazonian fishes more broadly. David Brooks is a recent Rome Prize recipient whose work explores the interaction between humans and their natural and synthetic environments. Several of his works have been influenced or inspired by his participation in scientific expeditions led by Nathan, this being the sixth that they have conducted together. Environmental Filmmaker Ivy Yin is a third collaborator who has now joined Nathan and David on two expeditions to the Amazon Basin.